This compound is primarily utilized in peptide synthesis, particularly in the production of custom peptides for research and pharmaceutical applications. It serves as a protected amino acid derivative, ensuring specific amino acids are incorporated correctly during the synthesis process. The tert-butyl (Tbu) group protects the hydroxyl group of threonine, while the N-methylation enhances stability and influences the peptide's conformational properties. This makes it valuable in designing peptides with improved bioavailability and resistance to enzymatic degradation, which is crucial in drug development and biochemical studies. Its application is especially significant in creating peptide-based therapeutics, such as hormones, enzyme inhibitors, and antimicrobial agents.