Used in peptide synthesis as a protected peptide fragment, enabling stepwise construction of complex peptides through solid-phase or solution-phase methods. The fluorenylmethyloxycarbonyl (Fmoc) group acts as a temporary protecting group for the N-terminus, allowing selective deprotection under mild basic conditions while preserving other functional groups. This compound serves as an intermediate in the preparation of biologically active peptides, particularly in pharmaceutical research and development. Its sequential assembly with other amino acids facilitates the study of structure-activity relationships in peptide-based drugs. Commonly applied in the synthesis of enzyme substrates, inhibitors, and peptide probes for biochemical assays.