GSK2334470 is primarily utilized in biochemical research as a potent and selective inhibitor of PDK1 (3-phosphoinositide-dependent protein kinase-1). It is widely used in studies aimed at understanding the role of PDK1 in cellular signaling pathways, particularly those related to cell growth, survival, and metabolism. Researchers employ GSK2334470 to investigate its effects on cancer cell proliferation and to explore potential therapeutic strategies targeting PDK1-dependent pathways. Additionally, it serves as a valuable tool in elucidating the mechanisms of insulin signaling and glucose metabolism, providing insights into metabolic disorders such as diabetes. Its application extends to preclinical studies where it helps in evaluating the efficacy of PDK1 inhibition in various disease models.