EvaGreen 2X qPCR MasterMix-iCycler is widely used in quantitative polymerase chain reaction (qPCR) applications for accurate and sensitive detection of DNA amplification. It is particularly valued for its compatibility with iCycler instruments, ensuring reliable performance in real-time PCR experiments. The dye-based system allows for monitoring of PCR products without the need for probes, making it cost-effective and easy to use. It is commonly applied in gene expression analysis, genotyping, and pathogen detection due to its high specificity and low background fluorescence. The mastermix is also suitable for high-throughput screening and research studies requiring precise quantification of nucleic acids. Its stability and consistency make it a preferred choice for laboratories focusing on molecular biology and diagnostics.