This buffer is primarily used in molecular biology for the annealing of single-stranded DNA oligos to form double-stranded DNA. It is essential in processes such as PCR, where primers need to anneal to the target DNA sequence for amplification. The buffer ensures optimal conditions for the hybridization of complementary DNA strands, providing the right ionic strength and pH. It is also utilized in the preparation of DNA probes for hybridization experiments, where precise annealing is critical for accurate binding to target sequences. Additionally, it supports the formation of DNA nanostructures in synthetic biology applications, facilitating the self-assembly of DNA into complex structures. Its consistent performance makes it a reliable tool in various DNA manipulation techniques.