10X Taq Buffer (with Mg2+) is widely used in molecular biology for PCR (Polymerase Chain Reaction) applications. It provides the optimal conditions for Taq DNA polymerase activity, ensuring efficient DNA amplification. The buffer stabilizes the pH and supplies essential magnesium ions, which act as cofactors for the enzyme. It is commonly employed in various PCR techniques, including gene cloning, mutagenesis, and diagnostic testing. Additionally, it is utilized in quantitative PCR (qPCR) and reverse transcription PCR (RT-PCR) for accurate and reliable results. Its ready-to-use formulation simplifies experimental setup and enhances reproducibility in DNA amplification processes.