PARP10-IN-3 is a potent and selective inhibitor of the PARP10 enzyme, which plays a role in cellular processes such as DNA repair, immune signaling, and cancer progression. Its primary application lies in cancer research, where it is used to study the effects of PARP10 inhibition on tumor cell growth and survival. By blocking PARP10 activity, this compound helps researchers understand the enzyme’s involvement in DNA damage response pathways and its potential as a therapeutic target in oncology.

It is also used in immunology research due to PARP10's role in regulating NF-κB signaling, a key pathway in inflammation and immune response. PARP10-IN-3 enables scientists to probe how modulation of this pathway affects immune cell function and inflammatory diseases.

Due to its selectivity, PARP10-IN-3 is a valuable tool for validating PARP10-specific effects without significant off-target inhibition commonly seen with broader PARP inhibitors. This makes it useful in target validation studies and in the development of more effective, targeted therapies for cancer and chronic inflammatory conditions.