4'-Hydroxyacetophenone oxime is primarily used in organic synthesis as an intermediate for the production of various pharmaceuticals and fine chemicals. It plays a significant role in the synthesis of heterocyclic compounds, which are essential in drug development. Additionally, it is utilized in the preparation of oxime ethers, which have applications in medicinal chemistry due to their biological activity. This compound is also employed in analytical chemistry as a reagent for the detection and quantification of specific metal ions, leveraging its chelating properties. Its versatility makes it valuable in research and industrial processes.