5(S)-HETrE is primarily used in biochemical research to study its role in inflammatory processes and cellular signaling. It serves as a key intermediate in the arachidonic acid pathway, helping researchers understand the mechanisms of lipid mediators in immune responses. Additionally, it is utilized in the development of anti-inflammatory drugs and therapies targeting conditions like asthma, arthritis, and other inflammatory diseases. Its application extends to exploring its effects on vascular and bronchial tissues, providing insights into cardiovascular and respiratory health.