3-Nitrotyramine is primarily used in biochemical research as a marker for oxidative stress and nitrosative damage in biological systems. It is formed through the nitration of tyrosine residues in proteins, a process often associated with the presence of reactive nitrogen species like peroxynitrite. Researchers utilize 3-Nitrotyramine to study the mechanisms of cellular damage in various diseases, including neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions. Its detection helps in understanding the role of oxidative stress in disease progression and in evaluating the efficacy of antioxidants or therapeutic agents aimed at mitigating such damage. Additionally, it serves as a reference compound in analytical methods like mass spectrometry and chromatography for identifying and quantifying nitrated proteins in complex biological samples.