Used to detect superoxide anion radicals in plant tissues, this staining solution helps visualize sites of reactive oxygen species (ROS) production under stress conditions such as drought, pathogen attack, or heavy metal exposure. The solution infiltrates plant cells and reacts with superoxide to form a blue formazan precipitate, allowing researchers to locate and semi-quantify oxidative stress responses. It is commonly applied in physiological and pathological studies to assess plant health, defense mechanisms, and the effectiveness of antioxidant systems. The method is widely used in plant pathology, environmental stress research, and genetic studies involving ROS-scavenging enzymes like superoxide dismutase.