The Total Antioxidant Capacity (T-AOC) Assay Kit (TPTZ) is widely used in research and clinical settings to measure the overall antioxidant capacity of biological samples. It is particularly valuable in studying oxidative stress, which is linked to various diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions. By quantifying the ability of antioxidants to reduce ferric ions to ferrous ions in the presence of TPTZ, this kit provides insights into the antioxidant defense mechanisms of cells and tissues. It is commonly applied in food science to evaluate the antioxidant properties of beverages, supplements, and food products. Additionally, it is used in pharmacology to assess the efficacy of antioxidant drugs and therapies. The kit is also employed in environmental studies to analyze the oxidative stress responses in organisms exposed to pollutants. Its simplicity, sensitivity, and reliability make it a preferred tool for antioxidant capacity assessment across diverse fields.