The Total Antioxidant Capacity (T-AOC) Assay Kit (TPTZ) is widely used in research and clinical settings to measure the overall antioxidant capacity of biological samples. It is particularly valuable in evaluating the ability of plasma, serum, tissue homogenates, and cell lysates to neutralize free radicals and oxidative stress. This kit is frequently employed in studies related to aging, inflammation, and diseases such as diabetes, cardiovascular disorders, and neurodegenerative conditions, where oxidative damage plays a critical role. Additionally, it is used in food science to assess the antioxidant properties of beverages, supplements, and food products, ensuring their quality and health benefits. The TPTZ method is favored for its sensitivity, reproducibility, and ability to provide a comprehensive measure of antioxidant activity across various sample types.