Used to measure the total sulfhydryl group content in biological samples, providing insights into oxidative stress and redox status. Commonly applied in research involving proteins, peptides, and enzymes to assess thiol group levels, which are critical for maintaining cellular function and antioxidant defense. Useful in studies related to aging, disease mechanisms, and drug development, where oxidative damage is a key factor. The kit is also employed in food science to evaluate the quality and stability of protein-rich products by monitoring thiol oxidation. Simple and efficient, it is a valuable tool for both academic and industrial laboratories.