Bilirubin oxidase is widely used in medical diagnostics, particularly for measuring bilirubin levels in blood samples. It helps in diagnosing liver disorders and jaundice by converting bilirubin into biliverdin, which can be easily quantified. In biosensors, it enhances the detection of bilirubin with high specificity and sensitivity. Additionally, it is employed in biofuel cells as a biocatalyst for oxygen reduction, improving energy conversion efficiency. Its application extends to environmental monitoring, where it aids in detecting and degrading bilirubin in wastewater. The enzyme’s ability to function under mild conditions makes it valuable in various biotechnological processes.