Used as a fluorescent dye in biological staining and microscopy due to its strong emission under UV or visible light. Commonly applied in cell imaging to visualize cellular structures, especially in lysosomes and acidic organelles, because of its pH-sensitive fluorescence. Also utilized in forensic science for fingerprint detection on porous surfaces, where it binds to amino acids and reveals latent prints with high contrast. Its photostability and high quantum yield make it suitable for long-term imaging and fluorescence-based assays.