Trimethylamine N-oxide dihydrate is widely used in biological and chemical research as a stabilizing agent for proteins and enzymes. It helps maintain the native structure of proteins during experiments, particularly in studies involving denaturation or folding processes. In the field of biochemistry, it is employed as an osmolyte, protecting cells and cellular components from stress caused by extreme environmental conditions such as high salinity or temperature. Additionally, it serves as a methyl donor in various metabolic pathways, making it valuable in studies of cellular metabolism and biochemical reactions. Its role in marine biology is also notable, as it is involved in the osmoregulation of marine organisms, helping them adapt to their saline environments.