O-Butyryltimolol is primarily utilized in ophthalmic applications for the treatment of glaucoma and ocular hypertension. It functions as a beta-adrenergic blocker, reducing intraocular pressure by decreasing the production of aqueous humor in the eye. This helps in preventing optic nerve damage and preserving vision in patients with these conditions. Additionally, it may be used in research settings to study the effects of beta-blockers on ocular tissues and their mechanisms of action. Its specific formulation ensures targeted delivery and efficacy in managing eye-related disorders.