4'-Demethylpodophyllotoxin is primarily used in the development of anticancer drugs due to its ability to inhibit cell division by interfering with microtubule formation. It serves as a key intermediate in the synthesis of etoposide and teniposide, which are widely used in chemotherapy for treating various cancers, including lung cancer, testicular cancer, and lymphomas. Additionally, its antiviral properties make it a potential candidate for research in antiviral therapies. Its role in inducing apoptosis in cancer cells further highlights its significance in oncology research and drug development.