Used in oncology research as an intermediate in the synthesis of pemetrexed, an antifolate agent employed in chemotherapy. It plays a role in the preparation of active pharmaceutical ingredients for the treatment of non-small cell lung cancer and mesothelioma. Its ester form enhances permeability across cell membranes, improving intracellular delivery before conversion to the active metabolite. Commonly utilized in formulation development and process optimization in drug manufacturing.