Used in research as a prodrug or intermediate in the development of antifolate agents, particularly in oncology. It targets folate metabolism in rapidly dividing cells, showing potential in cancer therapy. The ester groups enhance cellular uptake, allowing for improved delivery of the active compound. After entry into cells, the esters are hydrolyzed to release the active drug, which inhibits key enzymes involved in nucleotide synthesis. This compound is mainly utilized in preclinical studies to evaluate efficacy, metabolism, and toxicity of novel antitumor agents.