Used in targeted cancer therapies, particularly as a folate receptor-targeting agent in antitumor drug conjugates. Its structure enables selective delivery of cytotoxic agents to cancer cells overexpressing folate receptors, enhancing treatment specificity and reducing damage to healthy tissues. Commonly employed in the development of antibody-drug conjugates (ADCs) and prodrugs for improved pharmacokinetics and tumor uptake. Also utilized in research settings for studying folate-mediated cellular uptake mechanisms.