Used in research to selectively inhibit the HIF-2α transcription factor, HIF-2α-IN-3 is valuable for studying hypoxia signaling pathways. It shows potential in targeting cancers where HIF-2α is overactive, especially clear cell renal cell carcinoma. The compound helps block tumor growth by disrupting the interaction between HIF-2α and its partner protein HIF-1β. It is also employed in exploring metabolic and angiogenic processes regulated by oxygen levels in cells.