Used as a starting material in the synthesis of drugs related to cancer treatment, particularly those that inhibit the dihydrofolate reductase (DHFR) enzyme, which plays a key role in the nucleic acid synthesis pathway of cancer cells. It helps reduce abnormal cell proliferation. This compound is utilized in developing highly effective chemotherapy agents with reduced side effects compared to traditional drugs in the class. Additionally, it is employed in biochemical research to study folate metabolism pathways in animal and human cells.