JQ-1 carboxylic acid is primarily utilized in biomedical research, particularly in the study of epigenetic regulation and protein-protein interactions. It serves as a key intermediate in the synthesis of BET bromodomain inhibitors, which are crucial for investigating gene expression and chromatin remodeling. This compound is instrumental in developing therapeutic agents targeting cancers, inflammatory diseases, and other conditions linked to dysregulated transcription. Its application extends to drug discovery, where it aids in optimizing the potency and selectivity of small-molecule inhibitors. Additionally, JQ-1 carboxylic acid is used in biochemical assays to explore the mechanisms of bromodomain-containing proteins, contributing to advancements in understanding cellular signaling pathways.