ONO-AE3-208 is primarily used in research settings to study the role of prostaglandin E2 (PGE2) receptors, specifically the EP3 receptor, in various physiological and pathological processes. It acts as a selective antagonist for the EP3 receptor, making it a valuable tool for understanding the receptor's function in inflammation, pain, and cardiovascular regulation. Researchers utilize it to explore its potential therapeutic applications in conditions like chronic pain, thrombosis, and hypertension. Additionally, it helps in investigating the receptor's involvement in cancer progression and immune response modulation. Its specificity allows for targeted studies, contributing to the development of novel treatments for EP3 receptor-related diseases.