D-Stachyose is widely used in the food and beverage industry as a functional ingredient due to its prebiotic properties. It promotes the growth of beneficial gut bacteria, such as bifidobacteria, which supports digestive health and enhances nutrient absorption. In the pharmaceutical sector, it is utilized in formulations aimed at improving gut microbiota balance and managing gastrointestinal disorders. Additionally, D-Stachyose is incorporated into dietary supplements to boost immunity and overall wellness. Its natural sweetness and low caloric content also make it a suitable sugar alternative in health-focused food products. In research, it serves as a valuable tool for studying carbohydrate metabolism and gut microbiome interactions.