Adenosine 5'-diphosphate disodium salt is widely used in biochemical research as a substrate or intermediate in enzymatic reactions, particularly those involving ATPases, kinases, and other enzymes that regulate cellular energy metabolism. It plays a critical role in studying energy transfer processes within cells, making it valuable for understanding metabolic pathways and cellular functions. Additionally, it is employed in the development of diagnostic assays to measure enzyme activity or to investigate nucleotide-related biochemical mechanisms. Its stability and solubility in aqueous solutions make it suitable for laboratory experiments and in vitro studies.