Adenosine-3'-monophosphate is primarily used in biochemical research to study enzyme mechanisms and metabolic pathways. It serves as a substrate or intermediate in various enzymatic reactions, particularly those involving nucleotide metabolism. In molecular biology, it is utilized to investigate the role of nucleotides in cellular processes such as energy transfer and signal transduction. Additionally, it is employed in the synthesis of modified nucleotides for therapeutic and diagnostic applications. Its role in regulating cellular functions makes it valuable in studies related to cell signaling and metabolism.