Primarily utilized in biochemical research, this compound serves as a crucial substrate in enzymatic studies, particularly those involving kinases and phosphatases. It plays a significant role in investigating cellular signaling pathways, aiding in the understanding of phosphate group transfer mechanisms. Additionally, it is employed in the synthesis of modified nucleotides, which are essential for developing novel therapeutic agents and molecular probes. Its application extends to studying membrane-associated processes due to its structural similarity to naturally occurring phosphoester compounds. In drug discovery, it acts as a building block for designing inhibitors targeting specific enzymes involved in disease pathways.