Inosine 5'-triphosphate trisodium salt is widely used in biochemical and molecular biology research as a substrate for enzymes such as RNA polymerases and kinases. It plays a crucial role in studying nucleic acid synthesis and metabolism, particularly in understanding the mechanisms of transcription and replication. Additionally, it is employed in enzymatic assays to investigate nucleotide-dependent processes and to develop diagnostic tools for genetic disorders. In biotechnology, it serves as a component in the synthesis of modified RNA molecules, aiding in the development of therapeutic agents and vaccines. Its application extends to cell culture studies, where it helps in maintaining nucleotide pools and supporting cellular functions.