Pseudouridine-5′-triphosphate Sodium Salt is widely used in the field of molecular biology, particularly in the synthesis of modified RNA. It serves as a crucial component in the production of mRNA vaccines, where it enhances the stability and translation efficiency of the mRNA. This modification helps reduce the immunogenicity of the RNA, making it less likely to trigger an immune response when introduced into the body. Additionally, it is employed in research to study RNA structure and function, as well as in the development of therapeutic RNAs for treating various diseases. Its application extends to improving the efficacy and safety of RNA-based therapeutics and vaccines.