Used extensively in biochemical research to study enzyme kinetics, nucleotide metabolism, and the role of GTP-binding proteins such as G-proteins. It serves as a substrate or product in assays for enzymes like kinases and GTPases, aiding in the exploration of cellular signaling pathways. Derived from Saccharomyces cerevisiae, this tris salt form is particularly valuable in yeast-based studies to elucidate metabolic processes and energy transfer mechanisms in cellular systems.