Adenosine 2',5'-diphosphate sodium salt is primarily used in biochemical research to study enzyme mechanisms, particularly those involving nucleotide metabolism. It serves as a substrate or inhibitor in enzymatic assays, helping to elucidate the function of kinases, phosphatases, and other nucleotide-interacting enzymes. This compound is also valuable in investigating cellular signaling pathways, as it can mimic or interfere with natural nucleotide interactions. Additionally, it is utilized in the development of diagnostic tools and therapeutic agents targeting nucleotide-related processes. Its role in metabolic studies further aids in understanding energy transfer and regulation within cells.