Adenosine 5′-triphosphate disodium salt trihydrate is widely used in biochemical research as an energy source in cell metabolism studies. It plays a critical role in enzymatic reactions, particularly those involving kinases and ATPases, where it serves as a phosphate donor. This compound is also utilized in muscle physiology studies to understand energy transfer mechanisms during muscle contraction. Additionally, it is employed in molecular biology for processes like PCR and DNA ligation, where ATP is required as a cofactor. In cell culture applications, it supports energy-dependent processes, aiding in cell growth and viability. Furthermore, it is used in diagnostic assays to measure enzymatic activities and in drug discovery to screen potential therapeutic compounds targeting ATP-dependent pathways.