This chemical is widely used in molecular biology and biochemistry research as a substrate for RNA synthesis. It plays a crucial role in enzymatic reactions, particularly in the study of RNA polymerases and other nucleic acid-related enzymes. It is also employed in the preparation of RNA molecules for various experimental applications, including transcription assays and RNA sequencing. Additionally, it serves as a precursor in the synthesis of modified nucleotides, which are essential tools in studying RNA structure and function. Its application extends to cell culture studies, where it is used to investigate cellular metabolism and nucleotide incorporation processes.