This chemical is primarily utilized in biochemical research as a caged compound, allowing for precise control over the release of ATP in cellular processes. It is particularly valuable in studies involving muscle contraction, enzyme kinetics, and intracellular signaling pathways, where timed and localized ATP release is critical. The 2-nitrophenyl ethyl group acts as a photolabile protecting group, which can be cleaved upon exposure to UV light, releasing ATP. This property makes it an essential tool in photolysis experiments to investigate rapid biological responses. Additionally, it is used in neuroscience to study synaptic transmission and other ATP-dependent mechanisms in real-time. Its water-soluble trisodium salt form ensures compatibility with aqueous biological systems.