Used extensively in molecular biology research, particularly in the study of RNA modifications and translation processes. It serves as a crucial component in understanding the role of cap structures in mRNA stability and protein synthesis. Often employed in enzymatic assays to investigate the activity of RNA capping enzymes and decapping enzymes. Additionally, it aids in the development of therapeutic strategies targeting mRNA metabolism and gene expression regulation. Its application extends to the synthesis of modified nucleotides for advanced RNA-based technologies, including mRNA vaccines and RNA therapeutics.