Cytidine-5-triphosphate, disodium salt (CTP), is a ribonucleotide widely used in molecular biology and biochemistry research. It serves as a crucial substrate for RNA polymerases in the in vitro synthesis of RNA during transcription processes. This compound is essential in enzymatic reactions involving nucleic acid synthesis, such as the production of messenger RNA (mRNA), transfer RNA (tRNA), and other RNA species. CTP is also employed in studies of nucleic acid metabolism, enzyme kinetics, and nucleotide signaling pathways, providing insights into cellular functions and biochemical mechanisms. Its applications extend to the development of RNA-based therapeutics, vaccines, aptamers, and diagnostic tools in biotechnology and pharmaceutical industries.