Gemcitabine monophosphate is primarily used in cancer treatment, particularly for various types of solid tumors. It is a key component in chemotherapy regimens for pancreatic cancer, often combined with other drugs to enhance its efficacy. Additionally, it is employed in the treatment of non-small cell lung cancer, bladder cancer, and breast cancer. The compound works by inhibiting DNA synthesis, which prevents cancer cells from proliferating. Its monophosphate form allows for better cellular uptake and conversion into the active metabolite, gemcitabine triphosphate, which directly interferes with DNA replication. This targeted mechanism makes it a valuable option in slowing tumor growth and improving patient outcomes in specific cancer types.