Cytidine 5'-Triphosphate Disodium Salt Hydrate is widely used in molecular biology and biochemistry research. It serves as a substrate in enzymatic reactions, particularly in the synthesis of RNA during in vitro transcription experiments. This compound is essential in studies involving DNA and RNA polymerases, where it acts as a building block for nucleic acid synthesis. Additionally, it is utilized in cell culture applications to support cellular metabolism and energy transfer processes. Its role in nucleotide metabolism and signaling pathways makes it valuable in understanding cellular functions and developing therapeutic strategies.