Adenosine-5-triphosphate, disodium salt, is widely used in biochemical research as an energy source in enzymatic reactions. It plays a critical role in studies involving cellular metabolism, ATPase activity, and energy transfer processes. This compound is also utilized in molecular biology for DNA and RNA synthesis, where it acts as a substrate for polymerases. Additionally, it is employed in diagnostic assays to measure enzyme kinetics and in cell culture applications to support cellular energy demands. Its stability and solubility make it a preferred choice for in vitro experiments requiring ATP.