Uridine 5'-triphosphate trisodium salt hydrate is widely used in biochemical research as a substrate for RNA synthesis in enzymatic reactions. It plays a critical role in studying RNA polymerase activity and understanding transcription mechanisms. Additionally, it is utilized in cell culture applications to support nucleotide metabolism and promote cellular growth. This compound is also employed in diagnostic assays to detect and quantify enzymatic activities related to nucleic acid synthesis. Its stability and solubility make it a preferred choice for various in vitro experiments in molecular biology and biotechnology.