Adenosine-5-monophosphate monohydrate, free acid, is widely used in biochemical research as a precursor for the synthesis of nucleotides and nucleic acids. It plays a crucial role in studying cellular energy transfer processes, particularly in ATP-related metabolic pathways. In the pharmaceutical industry, it is utilized in the development of drugs targeting cardiovascular diseases, as it helps regulate blood flow and heart function. Additionally, it is employed in diagnostic assays to evaluate enzyme activities, such as adenylate kinase and creatine kinase. Its application extends to the food industry, where it serves as a flavor enhancer, contributing to the umami taste in various products.