Used as a key building block in DNA synthesis applications, particularly in polymerase chain reaction (PCR) and DNA sequencing. Serves as a substrate for DNA polymerases, enabling the incorporation of guanine nucleotides into growing DNA strands. Essential in molecular biology techniques such as site-directed mutagenesis, cloning, and next-generation sequencing. Also utilized in enzymatic assays involving nucleic acid synthesis and repair. Its triphosphate form provides the necessary energy for phosphodiester bond formation during DNA elongation. Commonly included in nucleotide mixtures for in vitro DNA amplification and labeling.