Used in biochemical and molecular biology research as a modified nucleotide analog. Serves as a substrate or inhibitor in enzyme assays involving kinases, phosphorylases, or ATP-dependent enzymes due to its thiophosphate group, which confers resistance to hydrolysis. Employed in studies of RNA synthesis and degradation, particularly to probe the mechanism of ribonucleases or to stabilize RNA intermediates. Also utilized in crystallography studies to examine enzyme active sites where ATP binding occurs, benefiting from the thiophosphate’s altered electronic properties for structural analysis.