Widely used in biochemical and molecular biology research, this compound serves as a critical energy source in enzymatic reactions, particularly in kinase and ligase assays. It is essential in protein phosphorylation studies, where it donates phosphate groups to target proteins. Commonly applied in ATP-dependent cellular processes simulation, including DNA repair, replication, and signal transduction pathways. Frequently used in luciferase-based bioluminescence assays for detecting microbial contamination or monitoring gene expression. Also utilized in cell-free systems for synthesizing biomolecules and studying metabolic pathways. Its solution form ensures immediate usability and precise dosing in sensitive experiments.