Widely used in biochemical research as a precursor for the synthesis of nucleotides and nucleic acids. It plays a crucial role in studying cellular energy transfer processes, particularly in ATP-related pathways. Commonly employed in enzymatic assays to investigate kinase and phosphatase activities. Utilized in the production of cyclic AMP (cAMP) for signaling pathway studies. Applied in cell culture media to support nucleotide metabolism. Also serves as a component in diagnostic kits for enzyme activity measurements. Its sodium salt form enhances solubility, making it suitable for various aqueous-based experiments.