Guanosine 5′-triphosphate tris salt is widely used in biochemical research as a critical substrate for enzymatic reactions, particularly those involving GTP-binding proteins and GTPases. It plays a vital role in studying signal transduction pathways, where it acts as an energy source and a molecular switch to regulate cellular processes. In molecular biology, it is employed in in vitro transcription and translation systems to synthesize RNA and proteins. Additionally, it is utilized in assays to investigate the function of enzymes like kinases and polymerases, providing insights into cellular metabolism and gene expression. Its stability and compatibility with various experimental conditions make it a valuable tool in life sciences research.