Used in the synthesis of oligonucleotides, particularly in solid-phase DNA synthesis, where it serves as a protected nucleoside building block. The trityl (Trt) group protects the 5'-hydroxyl, while the benzoyl (Bz) group protects the exocyclic amine, ensuring selective reactivity during the coupling steps. This compound is essential in the production of modified DNA sequences for research, diagnostics, and therapeutic applications, such as antisense oligonucleotides and gene editing tools. Its protective groups are removed under specific conditions to yield the desired DNA strand.